PUMA is a DIY open source portable 3D printed microscope with augmented reality, fluorescence, phase contrast, epi-illumination and other advanced features. It's main features and limitations are discussed. Chapters 0:00 Introduction 1:00 Background References 1:32 Portability features 3:17 Modularity and Upgradeability 4:55 Affordability Features 6:11 Introducing the OptArc online store 6:46 Motorised Z stage 7:03 Spatial Light Modulator 7:48 Augmented Reality HUD 8:52 Limitations 12:48 Concluding remarks SUPPORT PUMA ============ PUMA is an open source microscopy project. You can help support the project by: 1. Become a Patron: 2. Donate via PayPal: 3. Simply subscribing to this YouTube channel, like, comment and share these videos. 4. Spread the word - post the news about PUMA and link to this YouTube channel on your social media sites and any other outreach method (tell your friends, colleagues, students or teachers and lecturers about PUMA, for example). 5. Consider purchasing your optics and related supplies from our affiliated online optics store, ( ) --- FOR FURTHER INFORMATION ======================= 1. Open access write-up about PUMA on the FreeCAD blog: 2. The official PUMA GitHub page where you can download the specs and source files to build or customise your own PUMA system: 3. The scientific peer reviewed publication on PUMA that was published in 2021 in the Journal of Microscopy (a journal of the Royal Microscopical Society) available here: Thanks for your interest in the PUMA microscope system. PJT 21/07/21